{"id":13968,"date":"2013-05-01T12:01:51","date_gmt":"2013-05-01T04:01:51","guid":{"rendered":"https:\/\/blog.kohan.com.tw\/?p=13968"},"modified":"2021-03-31T13:43:06","modified_gmt":"2021-03-31T05:43:06","slug":"%e6%b2%89%e7%a9%8d%e7%89%a9%e4%b8%ad%e6%b1%9e%e7%89%a9%e7%a8%ae%e5%88%86%e6%9e%90%e6%b3%95%ef%bc%9a%e5%be%ae%e6%b3%a2%e8%90%83%e5%8f%96-hplc-vgafs","status":"publish","type":"post","link":"https:\/\/blog.kohan.com.tw\/%e5%85%83%e7%b4%a0%e5%88%86%e6%9e%90\/%e6%b2%89%e7%a9%8d%e7%89%a9%e4%b8%ad%e6%b1%9e%e7%89%a9%e7%a8%ae%e5%88%86%e6%9e%90%e6%b3%95%ef%bc%9a%e5%be%ae%e6%b3%a2%e8%90%83%e5%8f%96-hplc-vgafs\/","title":{"rendered":"\u6c89\u7a4d\u7269\u4e2d\u6c5e\u7269\u7a2e\u5206\u6790\u6cd5\uff1a\u5fae\u6ce2\u8403\u53d6\/HPLC-VGAFS"},"content":{"rendered":"

\u672c\u6587\u5c07\u5206\u4eab\u5229\u7528CEM Discover SP\u805a\u7126\u5f0f\u5fae\u6ce2\u5408\u6210\u5100<\/a>\uff0c\u642d\u914d\u9ad8\u6548\u6db2\u76f8\u5c64\u6790\u5100\u8207\u51b7\u84b8\u6c23\u539f\u5b50\u87a2\u5149\u5149\u8b5c\u5100\uff08HPLC-VGAFS)\uff0c\u61c9\u7528\u65bc\u6c89\u7a4d\u7269\u4e2d\u6c5e\u7269\u7a2e\u6fc3\u5ea6\u7684\u5206\u6790\uff0c\u80fd\u5f97\u5230\u5feb\u901f\u3001\u4fbf\u5229\u53ca\u7d55\u4f73\u7684\u56de\u6536\u7387\u3002<\/p>\n

\u4ee5\u805a\u7126\u5f0f\u5fae\u6ce2\u5408\u6210\u5100\u642d\u914dHPLC-VGAFS\u9032\u884c\u6c89\u7a4d\u7269\u7532\u57fa\u6c5e\u5206\u6790<\/h3>\n

\u6c5e\u5728\u74b0\u5883\u4e2d\u7684\u7684\u6bd2\u6027\u3001\u751f\u7269\u5316\u5b78\u53cd\u61c9\u53ca\u904b\u884c\u65b9\u5f0f\u8207\u5176\u7269\u7a2e\u578b\u614b\u6709\u975e\u5e38\u5927\u7684\u95dc\u9023\uff0c\u786b\u9178\u9e7d\u9084\u539f\u7d30\u83cc\u5177\u6709\u5c07\u7121\u6a5f\u6c5e\u7532\u57fa\u5316\u7684\u80fd\u529b\uff0c\u6240\u4ee5\u5728\u88ab\u6c5e\u6c61\u67d3\u7684\u6c89\u7a4d\u7269\u4e2d\u5bb9\u6613\u767c\u73fe\u7532\u57fa\u6c5e\uff0c\u800c\u7532\u57fa\u6c5e(MeHg+<\/sup>)\u6b63\u662f\u6c5e\u7269\u7a2e\u4e2d\u6bd2\u6027\u6700\u9ad8\u7684\u4e00\u7a2e\uff0c\u56e0\u6b64\u6e2c\u5b9a\u6c89\u7a4d\u7269\u4e2d\u7684\u7532\u57fa\u6c5e\u6fc3\u5ea6\u662f\u975e\u5e38\u91cd\u8981\u7684\u8ab2\u984c\uff0c\u672c\u7814\u7a76\u7684\u76ee\u7684\u662f\u958b\u767c\u7c21\u55ae\u3001\u5feb\u901f\u4e14\u7a69\u5065\u7684\u5206\u6790\u65b9\u6cd5\u4f86\u5206\u6790\u6c89\u7a4d\u7269\u4e2d\u7684\u7532\u57fa\u6c5e\uff0c\u672c\u6587\u63a2\u8a0e\u4ee5\u9ad8\u6548\u6db2\u76f8\u5c64\u6790\u5100\u8207\u51b7\u84b8\u6c23\u539f\u5b50\u87a2\u5149\u5149\u8b5c\u5100(HPLC-VGAFS)\u4f86\u9032\u884c\u6c89\u7a4d\u7269\u4e2d\u6c5e\u7269\u7a2e\u6fc3\u5ea6\u7684\u5206\u6790\uff0c\u53d60.1% (v\/v)\u76842-mercaptoethanol\u4f5c\u70ba\u8403\u53d6\u8a66\u5291\uff0c\u4f7f\u7528Discover SP\u805a\u7126\u5f0f\u5fae\u6ce2\u5408\u6210\u5100\u9032\u884c\u5fae\u6ce2\u8403\u53d6\u5f8c\uff0c\u5c07\u6a23\u54c1\u96e2\u5fc3\uff0c\u53d6\u4e0a\u5c64\u6db2\u6ce8\u5165HPLC-VGAFS\u7cfb\u7d71\u4e2d\u9032\u884c\u6c5e\u7269\u7a2e\u7684\u5206\u96e2\u8207\u6e2c\u5b9a\uff0c\u85c9\u7531\u9006\u76f8C18\u7ba1\u67f1\uff0c\u79fb\u52d5\u76f8\u9078\u64c73%(v\/v) acetonitrile\u6df7\u540860 mM ammonium acetate-acetic acid (pH 4.5) \u82070.1% (v\/v) 2-mercaptoethanol\uff0c\u4ee52.0mL\/min-1\u7684\u6d41\u901f\u9032\u884c\u5206\u6790\uff0c\u7532\u57fa\u6c5e(MeHg+<\/sup>)\u7684\u5075\u6e2c\u6975\u9650\u70ba0.58ppb\uff0c\u5e73\u5747\u56de\u6536\u7387\u70ba96.2%\u3002\u4e59\u57fa\u6c5e(EtHg+<\/sup>)\u5075\u6e2c\u6975\u9650\u70ba1.1ppb\uff0c\u5e73\u5747\u56de\u6536\u7387\u70ba88.7%\u3002\u7121\u6a5f\u6c5e(Hg2+<\/sup>)\u5075\u6e2c\u6975\u9650\u70ba0.48ppb\uff0c\u5e73\u5747\u56de\u6536\u7387\u70ba95.8%\u3002<\/p>\n<\/div>

\"mercury<\/span><\/div><\/div>
<\/div><\/div><\/div><\/div>

\u4f7f\u7528\u805a\u7126\u5f0f\u5fae\u6ce2\u5408\u6210\u5100\u9032\u884c\u6c89\u7a4d\u7269\u6a23\u54c1\u524d\u8655\u7406<\/h3>\n

\u6c89\u7a4d\u7269\u6a23\u54c1\u53d6\u81ea\u4e2d\u570b\u7018\u5dde\u5e02\u6c5f\u967d\u5340\u7684\u6cb1\u6c5f\uff0c\u51b7\u51cd\u4e7e\u71e5\u8655\u7406\u5f8c\uff0c\u7814\u78e8\u4e26\u7be9\u9078\u7c92\u5f91\u5c0f\u65bc63\u03bcm\u7684\u90e8\u5206\uff0c\u723e\u5f8c\u4ee518\u2103\u5132\u5b58\u65bc\u68d5\u8272\u73bb\u7483\u74f6\u4e2d\u3002CRM\u5247\u9078\u7528IAEA-405\u8207ERMCC580\u3002\u6a23\u54c1\u8403\u53d6\u4f7f\u7528CEM Discover SP\u805a\u7126\u5f0f\u5fae\u6ce2\u5408\u6210\u5100\u9032\u884c\uff0c\u6b64\u5fae\u6ce2\u52a0\u901f\u53cd\u61c9\u7cfb\u7d71\u4f7f\u7528\u975e\u63a5\u89f8\u5f0f\u7d05\u5916\u7dda\u6eab\u5ea6\u611f\u61c9\u5668\u4f86\u91cf\u6e2c\u6eab\u5ea6\uff0c\u6eab\u5ea6\u5075\u6e2c\u7bc4\u570d\u70ba0\u00b0C\u81f3300\u00b0C\uff0c\u4e26\u5177\u5099\u5e02\u5834\u4e0a\u6700\u9ad8\u7684\u5fae\u6ce2\u5bc6\u5ea6\uff0c\u53ef\u8b93\u53cd\u61c9\u901f\u7387\u63d0\u534710\u500d\u4ee5\u4e0a\u3002 \u53d60.2\u514b\u6a23\u54c1\uff0c\u6dfb\u52a0\u6a19\u6e96\u54c1\u5f8c\uff0c\u653e\u5165\u5fae\u6ce2\u53cd\u61c9\u74f6\u4e2d\uff0c\u52a0\u51653mL\u76840.1% (v\/v) 2-mercaptoethanol\u8207\u652a\u62cc\u5b50\uff0c\u653e\u5165Discover SP\u4e2d\uff0c\u4ee5\u9032\u884c2\u5206\u9418\u9810\u652a\u62cc\u5f8c\uff0c\u4ee540\u2103\u6046\u6eab8\u5206\u9418\uff0c\u5f85\u5fae\u6ce2\u7d50\u675f\u4e26\u51b7\u537b\u5f8c\uff0c\u5c07\u6a23\u54c1\u4ee51500rpm\u96e2\u5fc33\u5206\u9418\uff0c\u518d\u4ee50.22\u03bcm\u7684\u73bb\u7483\u7e96\u7dad\u6ffe\u7d19\u904e\u6ffe\u4e26\u5132\u5b58\u65bc2mL vial\u4e2d\uff0c\u4fdd\u5b58\u65bc4\u2103\u7684\u51b0\u7bb1\u5167\u3002<\/p>\n<\/div>

<\/a><\/span><\/div><\/div>
CEM Discover SP\u805a\u7126\u5f0f\u5fae\u6ce2\u5408\u6210\u5100<\/a><\/h6><\/h6><\/div>
<\/div><\/div><\/div><\/div>

\u4ee5HPLC\u2013VGAFS\u7cfb\u7d71\u5206\u6790\u6c5e\u5143\u7d20\u6fc3\u5ea6\u00a0<\/strong><\/h3>\n

\u4e0b\u5716\u5373\u70ba\u672c\u5be6\u9a57\u4e2d\u4f7f\u7528\u7684HPLC\u2013VGAFS\u7cfb\u7d71\u8207\u5176\u7d30\u90e8\u689d\u4ef6\uff0c\u5305\u542b\u4e00\u53f0\u4f7f\u7528C18\u7ba1\u67f1\u4f86\u5206\u96e2\u6c5e\u7269\u7a2e\u7684HPLC\u7cfb\u7d71\u4ee5\u53ca\u5075\u6e2c\u5143\u7d20\u7269\u7a2e\u7684VGAFS\u7cfb\u7d71\u3002\u6a23\u54c1\u4ee5Rheodyne 7725i\u6a23\u54c1\u6ce8\u5c04\u95a5\u914d\u5408500\u03bcL\u6a23\u54c1\u56de\u8def\u7ba1\u6ce8\u5165\uff0c\u79fb\u52d5\u76f8\u5247\u4ee5Shimadzu LC-20AT HPLC\u5e6b\u6d66\u9032\u884c\u8f38\u9001\uff0c\u7ba1\u67f1\u9078\u7528150 mm \u00d7 4.6 mm\/5-\u03bcm\u7684Venusil MP-C18\u7ba1\u67f1(BonnaAgela Technologies Inc.)\uff0c\u4ee5isocratic\u6a21\u5f0f\u5728\u5ba4\u6eab\u4e0b\u9032\u884c\u5206\u96e2\uff0c\u5206\u96e2\u5f8c\u7684\u6c5e\u7269\u7a2e\u4ee5\u6fd5\u5f0f\u5316\u5b78\u6c27\u5316\u6cd5(K2<\/sub>S2<\/sub>O8<\/sub>)\u914d\u5408UV\u71c8(15W UV-C\u71c8\u7ba1\uff0c\u53f0\u7063Huadeng\u88fd)\uff0c\u65bc\u6c27\u5316\u53cd\u61c9\u7dda\u5708\u4e2d\u6c27\u5316\u70ba\u7121\u6a5f\u6c5e\u3002\u9084\u539f\u5291\uff08KBH4\uff09\u8207\u8f09\u9ad4\u6eb6\u6db2\uff08\u9e7d\u9178\uff09\u5247\u5728\u6a23\u54c1\u901a\u904e\u6c27\u5316\u53cd\u61c9\u7dda\u5708\u5f8c\u6ce8\u5165\uff0c\u518d\u9001\u5f80\u9084\u539f\u53cd\u61c9\u7dda\u5708\u3002\u6df7\u5408\u7269\u8207\u6c2c\u6c23\u6df7\u5408\u5f8c\u9001\u81f3\u6c23-\u6db2\u5206\u96e2\u88dd\u7f6e\uff0c\u6c23\u9ad4\u88ab\u5206\u96e2\u4e26\u9001\u5230\u539f\u5b50\u87a2\u5149\u5149\u8b5c\u5100\u4e2d(AFS-9130\uff0cTitan\u88fd)\uff0c\u5206\u6790\u6c5e\u5143\u7d20\u6fc3\u5ea6\u3002<\/p>\n<\/div>

<\/span><\/div><\/div>
\u88681. HPLC-VGAFS\u7cfb\u7d71\u689d\u4ef6<\/h6><\/h6><\/div>
<\/div><\/div><\/div><\/div>

\u4ee5\u805a\u7126\u5f0f\u5fae\u6ce2\u5408\u6210\u5100\u9032\u884c\u7532\u57fa\u6c5e\u524d\u8655\u7406\u4e4b\u5206\u6790\u7d50\u679c\u00a0<\/strong><\/h3>\n

\u8403\u53d6\u6eab\u5ea6\u6700\u4f73\u5316\u00a0<\/strong><\/h4>\n

\u8403\u53d6\u6642\u9593\u3001\u6eab\u5ea6\u4ee5\u53ca2-mercaptoethanol\u6fc3\u5ea6\u7686\u6703\u5f71\u97ff\u56de\u6536\u7387\u3002\u7d93\u6e2c\u8a66\u5f8c\uff0c\u9078\u64c78\u5206\u9418\u7684\u5fae\u6ce2\u8403\u53d6\u6642\u9593\u82070.1% (v\/v) 2-mercaptoethanol\u53ef\u5f97\u5230\u6700\u4f73\u7684\u56de\u6536\u7387\u3002\u4ee5\u5fae\u6ce28\u5206\u9418\u70ba\u689d\u4ef6\u9032\u884c\u4e0d\u540c\u6eab\u5ea6\u4e0b\u7684\u6e2c\u8a66\uff0c\u7d50\u679c\u53ef\u898b\u4e0b\u57161\u3002<\/p>\n<\/div>

<\/span><\/div><\/div>
\u57161\uff1a\u4ee5\u5fae\u6ce28\u5206\u9418\u70ba\u689d\u4ef6\u9032\u884c\u4e0d\u540c\u6eab\u5ea6\u4e0b\u7684\u8403\u53d6\u56de\u6536\u7387\u7d50\u679c<\/h6><\/h6><\/div>
<\/div><\/div><\/div><\/div>

\u6839\u64da\u6b64\u5716\uff0c\u53ef\u77e5\u9053\u96a8\u8457\u6eab\u5ea6\u753140\u2103\u589e\u52a0\u81f3120\u2103\uff0c\u4e59\u57fa\u6c5e\u7684\u56de\u6536\u7387\u753187.9%\u00b1 6.4% \u964d\u81f331.4% \u00b1 7.1%\u3002\u800c\u6eab\u5ea6\u753180\u2103\u589e\u52a0\u81f3120\u2103\u6642\uff0c\u7532\u57fa\u6c5e\u7684\u56de\u6536\u7387\u5247\u6703\u958b\u59cb\u4e0b\u964d\uff0c\u7136\u800c\uff0c\u6c5e\u96e2\u5b50\u7684\u56de\u6536\u7387\u537b\u6703\u56e0\u6b64\u800c\u5f9e92.3% \u00b1 5.3%\u81f3128.5% \u00b1 8.8%\uff0c\u9019\u4ee3\u8868\u7576\u6eab\u5ea6\u8f03\u9ad8\u6642\uff0c\u7532\u57fa\u6c5e\u8207\u4e59\u57fa\u6c5e\u6703\u958b\u59cb\u8f49\u63db\u6210\u6c5e\u96e2\u5b50\uff0c\u56e0\u6b64\u9078\u64c740\u2103\u4f5c\u70ba\u8403\u53d6\u6eab\u5ea6\u53ef\u964d\u4f4e\u7532\u57fa\u6c5e\u8207\u4e59\u57fa\u6c5e\u7684\u8f49\u63db\u7a0b\u5ea6\u3002<\/p>\n

\u8403\u53d6\u5206\u6790\u7d50\u679c<\/h4>\n

\u4ee52-mercaptoethanol\u9032\u884c\u6c89\u7a4d\u7269\u4e2d\u7684\u6c5e\u7269\u7a2e\u5206\u6790\u6709\u8a31\u591a\u512a\u52e2\uff0c\u5176\u8403\u53d6\u7a0b\u5e8f\u7c21\u55ae\u3001\u5feb\u901f\u4e14\u53ea\u4f7f\u7528\u6bd2\u6027\u8f03\u4f4e\u7684\u8403\u53d6\u8a66\u5291\u3002\u5206\u6790\u7d50\u679c\u5df2\u7d93\u5217\u65bc\u4e0b\u8868\uff0c\u4e26\u8207\u5176\u4ed6\u8403\u53d6\u65b9\u6cd5\u505a\u6bd4\u8f03\uff0c\u53ef\u770b\u51fa\u65b0\u8403\u53d6\u65b9\u5f0f\u7684\u56de\u6536\u7387\u8207\u820a\u7684\u65b9\u5f0f\u5341\u5206\u63a5\u8fd1\uff0c\u76f8\u5c0d\u6a19\u6e96\u504f\u5dee\u57288%\u4ee5\u5167\u3002<\/p>\n<\/div>

<\/span><\/div><\/div>
\u88682. CRM\u8207\u5be6\u969b\u6a23\u54c1\u4e4b\u6c5e\u7269\u7a2e\u56de\u6536\u7387\u6e2c\u8a66\uff08n=3\uff09<\/h6><\/h6><\/div>
<\/div><\/div><\/div><\/div>

\u4e0b\u8868\u5247\u986f\u793a\u51fa\u6b64\u5206\u6790\u65b9\u6cd5\u7684\u5075\u6e2c\u6975\u9650\uff0c\u4e26\u4ee5\u516d\u652f20ng\u6a23\u54c1\u9032\u884c\u4e86\u76f8\u5c0d\u6a19\u6e96\u504f\u5dee(RSD)\u8a08\u7b97\uff0c\u4e26\u53e6\u5916\u9032\u884c\u4e865\u300120\u53ca40ng\u56de\u6536\u7387\u7684\u6e2c\u8a66\uff0c\u56de\u6536\u7387\u7686\u76f8\u7576\u4e0d\u932f\u3002<\/p>\n<\/div>

<\/span><\/div><\/div>
\u88683. HPLC-VGAFS\u5206\u6790\u6548\u80fd<\/h6><\/h6><\/div>
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\u5fae\u6ce2\u8403\u53d6\u88dd\u7f6e\u80fd\u5927\u5e45\u63d0\u5347\u5206\u6790\u524d\u8655\u7406\u6548\u7387<\/h3>\n

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  • l\u00a0\u00a0 Speciation Analysis of Mercury in Sediments by HPLC Using HPLC Hyphenated to Vapour Generation Atomic Fluorescence Spectrometry Following Microwave-Assisted Extraction,Geng Leng, Li Feng, Shao-Bo Li, De-Zhong Dan, Ping Yang , May 01, 2013, LCGC Europe, Volume 26, Issue 5<\/li>\n<\/ul>\n<\/div>
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