{"id":15927,"date":"2022-08-08T12:36:33","date_gmt":"2022-08-08T04:36:33","guid":{"rendered":"https:\/\/blog.kohan.com.tw\/?p=15927"},"modified":"2023-09-18T14:55:20","modified_gmt":"2023-09-18T06:55:20","slug":"%e5%a6%82%e4%bd%95%e9%81%b8%e6%93%87%e5%90%88%e9%81%a9%e7%9a%84%e7%b4%b0%e8%83%9e%e5%ad%98%e6%b4%bb%e6%b8%ac%e8%a9%a6%e6%b3%95","status":"publish","type":"post","link":"https:\/\/blog.kohan.com.tw\/%e5%8c%96%e5%ad%b8%e5%90%88%e6%88%90-%e7%94%9f%e7%89%a9%e7%a7%91%e6%8a%80\/%e5%a6%82%e4%bd%95%e9%81%b8%e6%93%87%e5%90%88%e9%81%a9%e7%9a%84%e7%b4%b0%e8%83%9e%e5%ad%98%e6%b4%bb%e6%b8%ac%e8%a9%a6%e6%b3%95\/","title":{"rendered":"\u5982\u4f55\u9078\u64c7\u5408\u9069\u7684\u7d30\u80de\u5b58\u6d3b\u6e2c\u8a66\u6cd5?"},"content":{"rendered":"

\u4ec0\u9ebc\u662f\u7d30\u80de\u5b58\u6d3b\u7387\uff1f<\/h3>\n

\u7d30\u80de\u5b58\u6d3b\u7387\u662f\u6e2c\u91cf\u4e00\u500b\u7d30\u80de\u7fa4\u9ad4\u4e2d\u6d3b\u7d30\u80de\u6240\u5360\u7684\u6bd4\u4f8b\u3002\u5b58\u6d3b\u7387\u662f\u5c07\u6a23\u54c1\u4e2d\u7684\u6d3b\u7d30\u80de\u7e3d\u6578\u8207\u6b7b\u7d30\u80de\u7e3d\u6578\u76f8\u6bd4\u6240\u5f97\u5230\u7684\u767e\u5206\u6bd4\u3002\u7576\u5b58\u6d3b\u7387\u8d8a\u9ad8\uff0c\u4ee3\u8868\u6a23\u54c1\u4e2d\u6240\u542b\u6709\u7684\u6d3b\u7d30\u80de\u6578\u8d8a\u591a\u3002<\/p>\n

\u70ba\u4ec0\u9ebc\u8981\u6e2c\u91cf\u7d30\u80de\u5b58\u6d3b\u7387\uff1f<\/h3>\n

\u8a31\u591a\u5be6\u9a57\u57fa\u790e\u90fd\u8ddf\u7d30\u80de\u5b58\u6d3b\u7387\u6e2c\u91cf\u606f\u606f\u76f8\u95dc\uff0c\u50cf\u662f\u7d30\u80de\u57f9\u990a\u5be6\u9a57\u4e2d\u9700\u8981\u78ba\u8a8d\u4e0d\u540c\u6642\u9593\u9ede\u7d30\u80de\u7684\u6578\u91cf\u8207\u72c0\u614b\uff0c\u4ee5\u78ba\u4fdd\u5be6\u9a57\u80fd\u5920\u9806\u5229\u9032\u884c\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u5728\u85e5\u7269\u958b\u767c\u7684\u904e\u7a0b\u4e2d\uff0c\u7d30\u80de\u5b58\u6d3b\u7387\u4e5f\u6642\u5e38\u88ab\u7528\u4f86\u8a55\u4f30\u85e5\u7269\u5c0d\u4e0d\u540c\u7d30\u80de\u9020\u6210\u7684\u5f71\u97ff\uff0c\u6642\u5e38\u7576\u4f5c\u907f\u514d\u8f49\u79fb\u53ca\u65b0\u6cbb\u7642\u65b9\u6cd5\u7684\u7814\u7a76\u5de5\u5177\u3002<\/p>\n

\u5e02\u9762\u4e0a\u91cf\u6e2c\u7d30\u80de\u5b58\u6d3b\u7387\u7684\u65b9\u6cd5\u8207\u5de5\u5177\u6709\u54ea\u4e9b\uff1f<\/h3>\n

\u4ee5\u64cd\u4f5c\u65b9\u5f0f\u505a\u5340\u5206\uff0c\u53ef\u5206\u70ba\u624b\u52d5(\u4eba\u5de5)\u8207\u81ea\u52d5\u8a08\u6578\u3002<\/p>\n

\u624b\u52d5\u65b9\u5f0f\u9700\u4ee5\u4eba\u5de5\u5b8c\u6210\u67d3\u8272\u3001\u8a66\u5291\u6dfb\u52a0\u7b49\u6b65\u9a5f\u5f8c\uff0c\u5728\u5408\u9069\u8a2d\u5099\u8f14\u52a9\u4e0b\u5206\u6790\u6216\u8a08\u7b97\u6578\u91cf\uff0c\u518d\u4f9d\u5206\u6790\/\u8a08\u6578\u7d50\u679c\u63db\u7b97\u51fa\u7d30\u80de\u61f8\u6d6e\u6db2\u6fc3\u5ea6\u4e26\u6c42\u51fa\u5b58\u6d3b\u8207\u6b7b\u4ea1\u7d30\u80de\u6578\u7684\u6bd4\u4f8b\u3002<\/p>\n

\u81ea\u52d5\u8a08\u6578\u5247\u662f\u85c9\u7531\u6a5f\u5668\u7684\u8f14\u52a9\uff0c\u7c21\u5316\u6e2c\u5b9a\u904e\u7a0b\u4e2d\u4eba\u529b\u7684\u6295\u5165\uff0c\u7d93\u7531\u6a5f\u5668\u81ea\u52d5\u8a08\u7b97\u7d50\u679c\u3002<\/p>\n

\u82e5\u4ee5\u6e2c\u5b9a\u7684\u539f\u7406\u4f86\u5340\u5206\uff0c\u5247\u53ef\u5927\u81f4\u5206\u70ba\u5169\u985e\uff1a<\/p>\n

1. \u5c08\u6ce8\u6b7b\u7d30\u80de\u65b9\u6cd5\uff1a<\/p>\n

\u53f0\u76fc\u85cd\uff08trypan blue\uff09\u662f\u5e38\u7528\u65bc\u5340\u5206\u6d3b\u7d30\u80de\u8207\u6b7b\u7d30\u80de\u7684\u751f\u7269\u67d3\u8272\u5291\u3002\u5176\u67d3\u8272\u6cd5\u70ba\u67d3\u6599\u6392\u65a5\u8a66\u9a57\uff08dye exclusion\uff09\u85c9\u7531\u67d3\u5291\u80fd\u5920\u6ef2\u5165\u6b7b\u7d30\u80de\uff0c\u4f46\u6703\u88ab\u6d3b\u7d30\u80de\u7684\u7d30\u80de\u819c\u963b\u9694\u7684\u7279\u6027\uff0c\u52a0\u4ee5\u5340\u5206\u4ee5\u5229\u9032\u884c\u5b58\u6d3b\u7387\u7684\u8a08\u7b97\u3002\u7136\u800c\uff0c\u5373\u4f7f\u6709\u4e9b\u7d30\u80de\u4ecd\u662f\u6d3b\u7684\u4f46\u5df2\u5931\u53bb\u529f\u80fd\uff0c\u537b\u6709\u53ef\u80fd\u4e0d\u6703\u906d\u5230\u67d3\u8272\u3002\u4ee5\u5f80trypan blue\u67d3\u8272\u5f8c\u7684\u6a23\u54c1\u6703\u900f\u904e\u986f\u5fae\u93e1\u89c0\u5bdf\u4e26\u4eba\u5de5\u5b8c\u6210\u8a08\u6578\uff0c\u73fe\u5728\u4ea6\u6709\u81ea\u52d5\u7d30\u80de\u8a08\u6578\u5668<\/a>\u80fd\u5920\u81ea\u52d5\u8fa8\u8b58\u5f71\u50cf\u4e26\u5b8c\u6210\u5206\u6790\u3002LDH Assay Kit\u5247\u662f\u4ee5\u5316\u5b78\u5448\u8272\u7684\u65b9\u5f0f\u9032\u884c\u6e2c\u5b9a\uff0c\u7d30\u80de\u7834\u640d\u5f8cLDH\u6703\u88ab\u91cb\u653e\u51fa\u7d30\u80de\u5916\u4e26\u5c07WST\u9084\u539f\u6210\u9ec3\u8272\u7522\u7269\uff0c\u56e0\u6b64\u6e2c\u91cf\u5448\u8272\u7684\u8b8a\u5316\u53ca\u53ef\u63db\u7b97\u51faLDH\u88ab\u91cb\u653e\u7684\u91cf\uff0c\u4f86\u63a8\u6e2c\u7d30\u80de\u6b7b\u4ea1\u7a0b\u5ea6\u3002<\/span><\/p>\n

2. \u5c08\u6ce8\u6d3b\u7d30\u80de\u7684\u65b9\u6cd5\uff1a<\/p>\n

\u4ee5\u5075\u6e2c\u7d30\u80de\u4ee3\u8b1d\u6d3b\u6027\uff0c\u4f8b\u5982\u6e2c\u91cf\u7c92\u7dda\u9ad4\u812b\u6c2b\u9176\uff08Dehydrogenase\uff09\u7684\u6d3b\u6027\uff0c\u9032\u800c\u53cd\u61c9\u7d30\u80de\u7684\u5b58\u6d3b\u7387\u3002<\/p>\n

\u4ee5MTT\u8a66\u9a57\u70ba\u4f8b\uff0cMTT\u6703\u88ab\u6d3b\u7d30\u80de\u7c92\u7dda\u9ad4\u8f49\u5316\u6210\u7532\u4436(formazan)\u6676\u9ad4\uff0c\u5229\u7528\u5438\u5149\u5ea6\u7684\u6e2c\u5b9a\u53bb\u8a55\u4f30\u7c92\u7dda\u9ad4\u7684\u6d3b\u8e8d\u7a0b\u5ea6\uff0c\u5373\u53ef\u63a8\u4f30\u51fa\u6d3b\u7d30\u80de\u6578\u91cf\u3002\u5176\u9748\u654f\u5ea6\u9ad8\uff0c\u4f46\u8f49\u5316\u5f8c\u7684\u7532\u4436\u6676\u9ad4\u4e0d\u6eb6\u65bc\u6c34\uff0c\u9700\u7d93\u6eb6\u89e3\u5f8c\u624d\u80fd\u6aa2\u6e2c\uff0c\u4e0d\u50c5\u589e\u52a0\u5de5\u4f5c\u91cf\uff0c\u9593\u63a5\u4e5f\u6703\u5f71\u97ff\u5be6\u9a57\u7684\u6e96\u78ba\u6027\u3002CCK-8\u5247\u662f\u6aa2\u6e2c\u7c92\u7dda\u9ad4\u9175\u7d20\u6d3b\u6027\uff0c\u85c9\u7531\u67d3\u5291\u88ab\u9175\u7d20\u9084\u539f\u5f8c\u7684\u984f\u8272\u8b8a\u5316\u4ee5\u63a8\u4f30\u5b58\u6d3b\u7684\u7d30\u80de\u91cf\u3002<\/span><\/p>\n

\u4e0a\u8ff0\u7684\u5169\u985e\u65b9\u6cd5\uff0c\u5747\u662f\u91dd\u5c0d\u7d30\u80de\u672c\u8eab\u6216\u7522\u7269\u9032\u884c\u67d3\u8272\u6216\u5448\u8272\u5f8c\u5206\u6790\u63db\u7b97\u51fa\u7d30\u80de\u5b58\u6d3b\u7684\u6bd4\u7387\uff0c\u96d6\u7136\u95dc\u6ce8\u7684\u5c0d\u8c61\u53ca\u5448\u8272\u65b9\u5f0f\u4e0d\u76e1\u76f8\u540c\uff0c\u4f46\u90fd\u9700\u8981\u5728\u6a23\u54c1\u4e2d\u52a0\u5165\u984d\u5916\u7684\u8a66\u5291\u65b9\u80fd\u6e2c\u91cf\u7d30\u80de\u6d3b\u529b\u3002\u7136\u800c\uff0c\u9019\u4e9b\u8a66\u5291\u6703\u9020\u6210\u7d30\u80de\u6bd2\u6027\uff0c\u4e14\u96a8\u8457\u5448\u8272\u6642\u9593\u589e\u52a0\uff0c\u6703\u9020\u6210\u6a23\u54c1\u4e2d\u6b7b\u7d30\u80de\u7684\u6578\u76ee\u6301\u7e8c\u589e\u52a0\uff0c\u53ef\u80fd\u9032\u800c\u5c0e\u81f4\u6e2c\u91cf\u7684\u8aa4\u5dee\u3002<\/p>\n

\u53ef\u4ee5\u5728\u4e0d\u4f7f\u7528\u984d\u5916\u8a66\u5291\u7684\u60c5\u6cc1\u4e0b\u6e2c\u91cf\u7d30\u80de\u5b58\u6d3b\u7387\u55ce\uff1f<\/h3>\n

\u5728\u50b3\u7d71\u986f\u5fae\u93e1\u4e0b\u8a08\u6578\u7d30\u80de\u6216\u4f7f\u7528\u574a\u9593\u81ea\u52d5\u7d30\u80de\u8a08\u6578\u5668<\/a>\uff0c\u9700\u4f9d\u9760\u6a23\u54c1\u5438\u6536\u5149\u5f8c\u6240\u5448\u73fe\u7684\u756b\u9762\u5f71\u50cf\u4f9b\u6e2c\u91cf\u9032\u884c\u3002\u7531\u65bc\u5927\u90e8\u5206\u7684\u751f\u7269\u6a23\u54c1\u5728\u4eba\u773c\u8fa8\u8b58\u4e0b\u96e3\u4ee5\u5448\u73fe\u51fa\u660e\u986f\u7684\u984f\u8272\u5c0d\u6bd4\uff0c\u5f80\u5f80\u9700\u8981\u85c9\u7531\u67d3\u8272\u6216\u5448\u8272\u7684\u65b9\u5f0f\u4f86\u5354\u52a9\u4f30\u7b97\u7d30\u80de\u5b58\u6d3b\u7387\u3002<\/p>\n

\u8a31\u591a\u751f\u7269\u6216\u91ab\u5b78\u7814\u7a76\u9818\u57df\u4e2d\uff08\u5982\u85e5\u7269\u958b\u767c\uff09\u9700\u8981\u91cd\u8907\u6e2c\u5b9a\u7d30\u80de\u6d3b\u6027\u3002\u7136\u800c\uff0c\u9577\u6642\u9593\u66b4\u9732\u5728\u8a66\u5291\u4e0b\uff0c\u53ef\u80fd\u9020\u6210\u7d30\u80de\u6bd2\u6027\uff0c\u5f9e\u800c\u5f71\u97ff\u4e86\u7d30\u80de\u5b58\u6d3b\u7387\u7684\u6e2c\u91cf\u6e96\u78ba\u5ea6\u3002<\/p>\n

\u5728\u7d30\u80de\u6b7b\u4ea1\u904e\u7a0b\u4e2d\u6703\u4f34\u96a8\u7d30\u80de\u578b\u614b\u767c\u751f\u5287\u70c8\u8b8a\u5316\uff0c\u4f8b\u5982\u9ad4\u7a4d\u8b8a\u5316\u6216\u7d30\u80de\u7834\u788e\uff1b\u76f8\u8f03\u65bc\u4e00\u822c\u5149\u5b78\u651d\u5f71\uff0c\u5168\u50cf\u986f\u5fae\u651d\u5f71\u6280\u8853\u53ef\u4ee5\u6355\u6349\u7d30\u80de\u6b7b\u4ea1\u904e\u7a0b\u4e2d\u7684\u578b\u614b\u53ca\u7d50\u69cb\u8b8a\u5316\u3002\u6d3b\u7d30\u80de\u5916\u89c0\u7279\u5fb5\u6709\u5b8c\u6574\u7684\u7d30\u80de\u819c\u6df1\u8272\u8f2a\u5ed3\u3002\u76f8\u53cd\u5730\uff0c\u6700\u521d\u6b7b\u7d30\u80de\u7684\u8868\u73fe\u70ba\u9ed1\u8272\u7269\u9ad4\uff0c\u96a8\u8457\u6642\u9593\u5230\u665a\u671f\u6642\uff0c\u7531\u65bc\u7d30\u80de\u819c\u7834\u88c2\u548c\u7d30\u80de\u8cea\u6ef2\u6f0f\u7b49\u904e\u7a0b\uff0c\u56e0\u800c\u9020\u6210\u7d30\u80de\u7684\u908a\u754c\u6a21\u7cca\u4e0d\u6e05\u3002<\/p>\n<\/div>

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\u6d3b\u7d30\u80de\uff1a<\/span><\/strong>\u6709\u5b8c\u6574\u7d30\u80de\u819c<\/span><\/h4><\/h6><\/div>
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\u7d30\u80de\u6b7b\u4ea1\uff1a<\/strong>\u7d30\u80de\u5167\u7684\u7279\u5fb5\u9010\u6f38\u4e0d\u660e\u986f\u4e14\u7d30\u80de\u908a\u754c\u6a21\u7cca<\/h4><\/h6><\/div>
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3D\u5168\u50cf\u986f\u5fae\u651d\u5f71(Digital holographic microscopy, DHM)\u6280\u8853<\/a>\u53ef\u5728\u514d\u4f7f\u7528\u67d3\u8272\u5291\uff08\u5448\u8272\u5291\uff09\u8f14\u52a9\u7684\u60c5\u6cc1\u4e0b\u6536\u96c6\u7d30\u80de\u578b\u614b\u7684\u5b8c\u6574\u8cc7\u8a0a\uff0c\u4e26\u7531\u96fb\u8166\u81ea\u52d5\u5b8c\u6210\u8fa8\u5225\u8207\u8a08\u7b97\u3002\u900f\u904e\u5149\u6e90\u675f\u7a7f\u904e\u6a23\u54c1\uff0c\u56e0\u4e0d\u540c\u7269\u9ad4\u7684\u6298\u5c04\u7387\u4e0d\u540c\uff0c\u6709\u4e9b\u5149\u7dda\u6703\u7522\u751f\u7e5e\u5c04\uff08\u82e5\u78b0\u5230\u6a23\u54c1\uff09\uff0c\u5176\u9918\u5149\u7dda\u5247\u6703\u76f4\u63a5\u7a7f\u900f\uff08\u7121\u78b0\u5230\u6a23\u54c1\uff09\u3002\u6700\u5f8c\u6a5f\u53f0\u5167\u7684\u76f8\u6a5f\u611f\u6e2c\u5668\u6703\u6536\u96c6\u6240\u6709\u7e5e\u5c04\u5149\u7dda\u8207\u7a7f\u900f\u5149\u7dda\uff0c\u7d93\u7531\u96fb\u8166\u81ea\u52d5\u8a08\u7b97\u7522\u751f\u5168\u50cf\u651d\u5f71\u5f71\u50cf\u5716\u3002<\/p>\n<\/div>

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\u7d93\u7531\u6536\u96c6\u7d30\u80de\u7acb\u9ad4\u7d50\u69cb\u5f71\u50cf\uff0c\u8207\u4ee5\u5f80\u7684\u5e73\u9762\u7d30\u80de\u5f71\u50cf\u89c0\u5bdf\u76f8\u6bd4\uff0c\u5305\u542b\u66f4\u591a\u6709\u95dc\u7d30\u80de\u6a23\u54c1\u8a0a\u606f\uff0c\u4f8b\u5982\u7d30\u80de\u819c\u7684\u5b8c\u6574\u6027\u548c\u86cb\u767d\u8cea\u542b\u91cf\u7684\u4e0d\u540c\u5c31\u6703\u9020\u62103D\u5168\u50cf\u986f\u5fae\u651d\u5f71\u5716\u7684\u5dee\u7570\uff0c\u9019\u4e5f\u662f\u70ba\u4ec0\u9ebc\u53ef\u4ee5\u53d6\u5f97\u66f4\u591a\u7d30\u80de\u8a0a\u606f\u7684\u539f\u56e0\u3002<\/p>\n<\/div>

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\u904e\u53bb\u6211\u5011\u5f9e\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\u5230\u7684\u7d30\u80de\u5f71\u50cf\u70ba\u5e73\u9762\u5f71\u50cf\uff0c\u4eba\u773c\u518d\u5f9e\u5e73\u9762\u5f71\u50cf\u9032\u884c\u5206\u6790\u4f5c\u696d\u3002\u5982\u4eca\uff0c\u6280\u8853\u4e0d\u65b7\u63d0\u5347\uff0c\u7372\u53d6\u7acb\u9ad4\u5f71\u50cf\u53ef\u4ee5\u5f97\u5230\u7d30\u80de\u66f4\u5b8c\u6574\u5730\u8cc7\u8a0a\uff0c\u518d\u8f14\u4ee5\u96fb\u8166\u53caAI\u4eba\u5de5\u667a\u6167\u6f14\u7b97\uff0c\u5f97\u4ee5\u8b93\u6574\u500b\u5206\u6790\u6d41\u7a0b\u66f4\u52a0\u5feb\u901f\u4e14\u81ea\u52d5\u5316\u3002<\/p>\n

fluidlab R-<\/a>300<\/a>\u6a5f\u53f0\u4f7f\u7528\u6df1\u5ea6\u795e\u7d93\u7db2\u8def(CNN)\u9032\u884c\u81ea\u52d5\u6f14\u7b97\uff0c\u6e96\u78ba\u7684\u5206\u6790\u8a55\u4f303D\u5168\u50cf\u986f\u5fae\u651d\u5f71\u6240\u7372\u53d6\u7684\u5f71\u50cf\u8cc7\u8a0a\u3002\u7576\u6a5f\u53f0\u7684\u76f8\u6a5f\u611f\u61c9\u5668\u53d6\u5f97\u5168\u50cf\u651d\u5f71\u5f71\u50cf\u5716\u5f8c\uff0c\u5167\u5efa\u96fb\u8166\u6703\u6839\u64da\u7d30\u80de\u7684\u5916\u89c0\u578b\u614b\u4e0d\u540c\u52a0\u4ee5\u5206\u8fa8\uff0c\u53d6\u5f97\u8a08\u6578\u7d30\u80de(\u65b9\u6846\u6a19\u8a3b\u8a08\u6578\u7d30\u80de)\u53ca\u5206\u8fa8\u6d3b\u7d30\u80de\u8207\u6b7b\u7d30\u80de(\u4ee5\u7da0\u8272\u53ca\u6a58\u8272\u6a19\u8a3b\u4e0d\u540c\u7a2e\u985e)\u3002\u6700\u5f8c\uff0c\u9664\u4e86\u80fd\u5920\u5f97\u5230\u7d30\u80de\u6578\u91cf\u7684\u6578\u64da\u7d50\u679c\uff0c\u5831\u544a\u9084\u9644\u52a0\u76f4\u65b9\u5716\u8207\u5f71\u50cf\u5716\u3002\u76f4\u65b9\u5716\u6703\u8a18\u9304\u5168\u5c3a\u5bf8\u7bc4\u570d\u7684\u7d30\u80de\u6578\u91cf\uff0c\u4e26\u4e14\u53ef\u624b\u52d5\u8abf\u6574\u7d30\u80de\u5c3a\u5bf8\u7bc4\u570d\uff0c\u4ee5\u4e86\u89e3\u4e0d\u540c\u7d30\u80de\u5927\u5c0f\u5167\u7684\u6d3b\u7d30\u80de\u8207\u6b7b\u7d30\u80de\u6578\u91cf\uff0c\u4e5f\u53ef\u85c9\u6b64\u6392\u9664\u7d30\u80de\u788e\u7247\u7b49\u5176\u4ed6\u5e72\u64fe\u9805\u3002<\/p>\n<\/div>

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\u8a08\u7b97\u7d30\u80de\u6578\u91cf<\/h4><\/h6><\/div>
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\u5206\u8fa8\u6d3b\u7d30\u80de\/\u6b7b\u7d30\u80de<\/h4><\/h6><\/div>
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\u56e0\u61c9\u5b78\u8853\u7814\u7a76\u8207\u7522\u696d\u74b0\u5883\u7684\u6539\u8b8a
<\/b><\/h3>\n

fluidlab R-300<\/a>\u6a5f\u53f0\u9664\u4e86\u53ef\u4ee5\u81ea\u52d5\u7d30\u80de\u8a08\u6578\u53ca\u5b58\u6d3b\u7387\u5206\u6790\uff0c\u9084\u914d\u6709\u514d\u8cbb\u4e14\u597d\u7528\u7684\u50b3\u8f38\u8edf\u9ad4\uff0c\u80fd\u5920\u5feb\u901f\u6574\u7406\u5be6\u9a57\u6578\u64da\u8207\u5716\u7247\u3002\u5927\u91cf\u7d30\u80de\u8a08\u6578\u8207\u5206\u6790\u5f8c\uff0c\u4e0d\u5fc5\u518d\u4e00\u4e00\u6574\u7406\u6578\u64da\u7d50\u679c\u8207\u7d30\u80de\u5f71\u50cf\uff0c\u53d7\u5230\u7814\u7a76\u4eba\u54e1\u9752\u775e\uff0c\u6578\u7bc7\u8ad6\u6587\u4ee5fluidlab R-300\u6a5f\u53f0\u767c\u8868\u8ad6\u6587\u3002<\/p>\n<\/div>

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Anne Marzi\u8207Kai Moritz Eder\u5728\u7814\u7a76\u4e2d<\/a>\u5c0d\u805a\u5408\u7269\u5948\u7c73\u8f09\u9ad4\u9032\u884c\u7d30\u80de\u6bd2\u6027\u6e2c\u8a66\uff0c\u7576\u4e2d\u904b\u7528\u5230fluidlab R-300<\/a>\u4ee5\u9032\u884c\u7d30\u80de\u8a08\u6578\uff0c\u5be6\u9a57\u7d50\u679c\u5177\u6709\u826f\u597d\u7684\u7cbe\u78ba\u6027\u53ca\u518d\u73fe\u6027\u8868\u73fe\u3002
<\/b><\/p>\n

\u4f86\u81ea\u5fb7\u570b\u7684\u7814\u7a76\u5718\u968a<\/a>\u7814\u767c\u6bd4\u8272\u5316\u5b78\u50b3\u611f\u5668\u4ee5\u5075\u6e2c\u751f\u7269\u6db2\u9ad4\u4e2d\u7684\u812f\u80fa\u9178\uff08Proline\uff09\uff0c\u7576\u4e2d\u904b\u7528\u5230fluidlab R-300<\/a>\u4ee5\u9032\u884c\u5b9a\u6027\u53ca\u5b9a\u91cf\u5206\u6790\uff0c\u8f03\u4ee5\u5f80\u7684\u65b9\u5f0f\u66f4\u5feb\u4e14\u66f4\u7cbe\u78ba\u3002<\/p>\n

fluidlab R-300<\/a>\u5c07\u7d30\u80de\u8a08\u6578\u8207\u5206\u5149\u5149\u5ea6\u8a08\u5169\u9805\u5206\u6790\u6280\u8853\u5408\u65bc\u4e00\u6a5f\uff0c\u6709\u6548\u63d0\u5347\u57fa\u790e\u5be6\u9a57\u6548\u7387\u3002\u7d30\u80de\u8a08\u6578\u5668\u529f\u80fd\u7684\u90e8\u5206\uff0c\u642d\u914d\u8afe\u8c9d\u723e\u7269\u7406\u734e\u6280\u8853-3D\u5168\u50cf\u986f\u5fae\u651d\u5f71\uff08Digital Holographic Microscopy\uff09\uff0c\u4e0d\u518d\u9700\u8981\u67d3\u8272\u8a66\u5291\uff0c\u4ee5\u66f4\u4e0d\u50c5\u9054\u5230\u7da0\u8272\u74b0\u4fdd\u7684\u65b9\u5f0f\u5206\u6790\u6a23\u54c1\uff0c\u4e14\u53ef\u4ee5\u7701\u53bb\u7b49\u5f85\u7684\u6642\u9593\u3002\u6b64\u5916\uff0c\u5728\u5206\u5149\u5149\u5ea6\u8a08\u7684\u8868\u73fe\u6548\u80fd\u4e0a\u53ef\u6aa2\u6e2c\u6ce2\u9577\u7bc4\u570d375nm\u5230700nm\u7684\u5168\u6ce2\u6bb5\u5149\u8b5c\u3002\u9ad8\u7a69\u5b9a\u3001\u514d\u6821\u6b63\u7684\u53ef\u651c\u5f0f\u8a2d\u8a08\uff0c\u4ea6\u53ef\u76f4\u63a5\u5e36\u81f3\u7d30\u80de\u623f\u6216\u7121\u83cc\u64cd\u4f5c\u53f0\u5167\u3002<\/span><\/p>\n<\/div>

\"Anvajo<\/a><\/span><\/div><\/div>
<\/div><\/div><\/div><\/div>
<\/div><\/div><\/div><\/div><\/div>

\u6b32\u4e86\u89e3\u66f4\u591a\u7684\u5100\u5668\u8cc7\u8a0a\u8207\u5be6\u9a57\u7d30\u7bc0\uff0c\u6b61\u8fce\u4f86\u96fb\u6d3d\u8a62 02-2728-2767\u6216 \u4f86\u4fe1<\/span><\/a><\/span>\u8207\u6211\u5011\u806f\u7e6b<\/p>\n<\/div>

<\/div><\/div><\/div>

\u6b32\u4e86\u89e3\u66f4\u591a\u7684\u5100\u5668\u8cc7\u8a0a\u8207\u5be6\u9a57\u7d30\u7bc0\uff0c\u6b61\u8fce\u4f86\u96fb\u6d3d\u8a62 02-2728-2767<\/a><\/span>\u6216 \u4f86\u4fe1<\/span><\/a><\/span>\u8207\u6211\u5011\u806f\u7e6b<\/p>\n<\/div>

<\/div><\/div><\/div>

\u53c3\u8003\u6587\u737b<\/h3><\/div>

<\/p>\n

<\/p>\n

\n
    \n
  • \n

    https:\/\/anvajo.com\/inspiration\/stain-free-viability-determination<\/p>\n<\/li>\n

  • \n

    Shokrzadeh, M., & Modanloo, M. (2017). An overview of the most common methods for assessing cell viability. Journal of Research in Medical and Dental Science, 5, 33-41.<\/span><\/p>\n<\/li>\n

  • \n

    Riss, Terry L., et al. “Cell viability assays.” Assay Guidance Manual [Internet]. Eli Lilly & Company and the National Center for Advancing Translational Sciences, 2016.<\/span><\/p>\n<\/li>\n

  • \n

    Aslant\u00fcrk, \u00d6zlem Sultan. In vitro cytotoxicity and cell viability assays: principles, advantages, and disadvantages. Vol. 2. InTech, 2018.<\/span><\/p>\n<\/li>\n

  • \n

    Kock WE. Nobel prize for physics: gabor and holography. Science. 1971;174(4010):674-675. doi:10.1126\/science.174.4010.674<\/span><\/p>\n<\/li>\n

  • \n

    Method: Staining-free Viability Explained. https:\/\/anvajo.com\/storage\/media\/documents\/1076\/fluidlab_viability_explained.pdf<\/span><\/p>\n<\/li>\n

  • \n

    Method explained: Holografic Analysis of Single Cells.<\/span><\/p>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>

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